SCNP Platform Technology
Impact of time from blood draw to peripheral blood mononuclear cell (PBMC) processing and cryopreservation as functional pathway activity measured by single cell network profiling (SCNP) assays.
Longo D, Louie B, Evensen E, Hawtin RE, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2011;118:Abstract 4922.
Sample cryopreservation does not affect functional read outs in SCNP assays: implications for biomarker development.
Cesano A, Gotlib J, Lacayo NJ, Putta S, Lackey A, Gayko U, and Kornblau SK.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 4843.
Informatics platform and workflows for robust high throughput single cell network profiling (SCNP).
Putta S, Spellmeyer D, Evensen E, Banville S, Friedland B, Rosen D, Soper D, Purvis N, Covey T, Francis-Lang H, and McCarthy J.
In: Program and Abstracts of the XXV Congress of the International Society for Advancement of Cytometry; 2010 May 8-12; Seattle, WA. Maiden (MA): Wiley; 2010. Abstract 43.
Reproducibility of single cell network profiling using multiparameter flow cytometry.
Evensen E, Palazzo A, Gulrajani M, Purvis N, Banville S, Putta S, Gayko U, and Fantl W.
In: Program and Abstracts of the XXV Congress of the International Society for Advancement of Cytometry; 2010 May 8-12; Seattle, WA. Maiden (MA): Wiley; 2010. Abstract 191.
Towards robust evaluation of gating in flow cytometry.
Putta S.
In: Program and Abstracts of the XXV Congress of the International Society for Advancement of Cytometry; 2010 May 8-12; Seattle, WA. Maiden (MA): Wiley; 2010. Abstract 192.
A comparative analysis of quantitative techniques using flow and mass cytometric platforms.
Purvis N, Ornatsky O, Shults K, Baranov V, Tanner, S, and Stelzer G.
In: Program and Abstracts of the XXV Congress of the International Society for Advancement of Cytometry; 2010 May 8-12; Seattle, WA. Maiden (MA): Wiley; 2010. Abstract 375.
Drug Development
Single-cell network profiling (SCNP) to evaluate the proteomic profiles associated with ON 01910.Na treatment of MDS patients (pts).
Cesano A, Soper D, Banville S, Tran M, Seetharam M, Jumar R, Wilhelm F, and Greenberg P.
In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; 2011. Abstract 4136.
Single cell network profiling (SCNP) of IFN-α signaling pathways in peripheral blood mononuclear cells from healthy donors: implications for disease characterization, treatment selection, and drug discovery.
Longo DM, Evensen E, Fantl WJ, Pos Z, Marincola F, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 4867.
Single cell network profiling (SCNP) to evaluate the mechanism of action of ON 01910.Na, a novel clinical trial stage compound.
Soper DM, Huang Y-W, Wilhelm F, Cosenza SC, Reddy EP, Cesano A, Greenberg PL, and Fantl WJ.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 3827.
Single cell network profiling (SCNP) by flow cytometry as a tool to measure potency and selectivity of JAK/STAT inhibitors in PBMC and whole blood discrete cell subsets.
Covey TM, Putta S, Gulrajani M, Cohen AC, and Cesano A.
Mol Cancer Ther. 2009;8:B182.
Acute Myeloid Leukemia
Single cell network profiling (SCNP)-based classifier to predict response to induction therapy in pediatric patients with de novo acute myeloid leukemia (AML): validation study results.
Lacayo NJ, Alonzo TA, Gayko U, Westfall M, Purvis N, Putta S, Hackett J, Cohen AC, Gerbing RB, Dahl GV, Bamis AS, and Meshinchi S.
Blood (ASH Annual Meeting Abstracts). 2011;118:Abstract 3544.
Single cell network profiling (SCNP) functionally characterizes FLT3 pathway deregulation in non-M3 acute myeloid leukemia (AML) and provides prognostic value independent from mutational status.
Cesano A, Putta S, Mathi K, Rosen DB, Gayko U, Hawtin RE, Cripe LD, Sun Z, Tallman MS, and Paietta E.
Blood (ASH Annual Meeting Abstracts). 2011;118:Abstract 2512.
Assessing specific biological mechanisms of drug resistance in adult and pediatric AML.
Rosen DB, Cordeiro J, Cohen AC, Lacayo N, Hogge D, Cesano A, and Hawtin R.
AACR International Conference: Molecular Targets and Cancer Therapeutics 2011 Nov 12-16; San Francisco, CA. Abstract # B72.
Integrating in vitro signaling and drug response data from single cell network profiling (SCNP) to inform on individual's AML biology.
Covey T, Gulrajani M, Hawtin R, Gayko U, Cumpston A, Craig M, Hamadani S, Rishel A, Park J, Abraham J, Remick S, and Tse W.
AACR International Conference: Molecular Targets and Cancer Therapeutics 2011 Nov 12-16; San Francisco, CA. Abstract # A73.
Using Single-cell Network Profiling (SCNP) signatures to predict response to induction therapy and relapse risk in paediatric patients with acute myeloid leukemia (AML).
Cesano A, Westfall M, Rosen D, Louie B, Meshinchi S, Alonzo T, Arceci R, Ravindranath Y, Dahl G, and Lacayo N.
EJC (The 2011 European Multidisciplinary Cancer Congress). 2011;47n(Suppl 1):Abstract 1144.
Single cell network profiling (SCNP) signatures predict response to induction therapy and relapse risk in pediatric patients with acute myeloid leukemia: Children's Oncology Group (COG) study POG-9421.
Lacayo NJ, Cohen A, Westfall M, Lackey A, Xin X, Gayko U, Putta S, Meshinchi S, Raimondi S, Alonzo TA, Arceci RJ, Ravindranath Y, Van Houten Dahl G, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 954.
Characterization of FLT3 pathway deregulation by single cell network profiling (SCNP) stratifies AML patients beyond molecular profiling.
Rosen DB, Putta S, Gayko U, Cesano A, Cripe L, Sun Z, Litzoe MR, Uno H, Tallman MS, and Paietta E.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 2739.
Single cell network profiling as a platform to reveal leukemia-specific signaling signatures and sensitivity to kinase inhibitor therapies.
Covey TM, Gulrajani M, Becker H, Chandler JC, Schwind S, Marcucci G, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 2753.
Single-cell network profiling (SCNP) signatures independently predict response to induction therapy in older patients with acute myeloid leukemia (AML).
Cesano A, Putta S, Gayko U, Hackett J, Rosen DB, Cripe L, Sun Z, Uno H, Litzow MR, Tallman MS, and Paietta E.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 2695.
Specimen source (BM or PB) does not affect proteomic signaling in patients with AML and circulating blasts.
Cesano A, Rosen DB, Putta S, Gayko U, Cripe L, Sun Z, Uno H, Litzow MR, Tallman MS, and Paietta E.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 2693.
Single cell network profiling (SCNP) offers a novel approach to identify at diagnosis AML chemotherapy resistant cell phenotypes.
Cohen A, Covey T, Putta S, Gayko U, Xiaohua X, Fantl W, Kornblau S, and Cesano A.
Haematologica. 2010;95(Suppl 2):Abstract 1167.
Biological characterization of AML at the single cell level based on response to DNA damaging agents.
Rosen D, Minden M, Putta S, Covey T, Huang Y-W, Cesano A, Nolan G, and Fantl W.
Haematologica. 2010;95(Suppl 2):Abstract 0041.
Dynamic single cell network profiles (SCNP) in AML are associated with patient response to standard induction therapy.
Cesano A, Minden M, Rosen D, Putta S, Cohen A, Fantl W, Gayko R, and Kornblau S.
Haematologica. 2010;95(Suppl 2):Abstract 0061.
Identification of distinct apoptosis and myeloid signaling profiles within acute myeloid leukemia (AML) blast subpopulations.
Rosen DB, Minden MD, Putta S, Covey T, Huang Y-W, Cesano A, Nolan GP, and Fantl WJ.
In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; 2010. Abstract 3842.
Classification of acute myeloid leukemia (AML) based on apoptosis and myeloid signaling networks.
Rosen DB, Minden MD, Putta S, Covey T, Huang YW, Cesano A, and Fantl WJ.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 325.
Single cell network profiling as a tool to identify AML chemotherapy resistant cell phenotypes under in vivo therapeutic pressure.
Cesano A, Woronicz J, Cohen AC, Covey T, Putta S, Gayko U, Fantl WJ, and Kornblau SM.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 397.
Single cell network profiles in non-M3 AML associated with patient response to standard induction therapy.
Kornblau SM, Minden MD, Rosen DB, Putta S, Cohen AC, Covey T, Fantl WJ, Gayko U, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 1582.
Flt3-ITD signaling profiles in AML samples harboring mutations.
Minden MD, Kornblau SM, Rosen DB, Cohen AC, Gayko U, Putta S, Woronicz J, Fantl WJ, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 1588.
Distinct signaling profiles of gemtuzumab ozogamicin responsiveness and refractoriness in acute myeloid leukemia.
Rosen DB, Cordeiro JA, Soper DM, Huang Y-W, Hogge DE, Walter RB, Cesano A, and Fantl WJ.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 2745.
Cost burden analysis of ineffective induction chemotherapy in elderly patients with AML.
Cohen AC, Davidson N, Scharf J, and Middlebrook D.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 4531.
Chronic Lymphocytic Leukemia
BCR responsiveness is associated with time to first treatment (TTFT) in B-cell chronic lymphocytic leukemia (B-CLL): results from a single cell network profiling (SCNP) verification study.
Cesano A, Evensen E, Ptacek J, Cordeiro J, Hawtin RE, Ware JR, Nichele I, and Scupoli MT.
Blood (ASH Annual Meeting Abstracts). 2011;118:Abstract 2834.
Single cell network profiling (SCNP) assay reveals B cell receptor signaling heterogeneity in the context of ZAP-70 expression in patients with chronic lymphocytic leukemia (CLL).
Shults K, Evensen E, Scupoli MT, Zanotti R, Xin X, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 3586.
BCR signaling profiles associated with prognosis and progression in B-CLL.
Scupoli MT, Perbellini O, Cioffi F, Chignola R, Evensen E, Zanotti R, Nichele I, Lovato O, and Pizzolo G.
Haematologica. 2010;95(Suppl 2):Abstract 0110.
Single cell profiling of B cell receptor signaling and apoptosis networks in chronic lymphocytic leukemia: association with in vitro sensitivity to fludarabine monophosphate (F-ara-A).
Evensen E, Palazzo A, Huang Y-W, Cesano A, Rassenti LZ, Kipps TJ, and Fantl WJ.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 1263.
High level phosphatase activity revealed in chronic lymphocytic leukemia cells that use mutated immunoglobulin heavy chain variable region genes and lack high-level expression of the zeta-associated protein 70 (ZAP-70).
Fantl WJ, Yafawi RL, Putta SK, Huang Y-W, Francis-Lang HL, Laura RZ, Cohen AC, and Kipps TJ.
Blood (ASH Annual Meeting Abstracts). 2007;110:Abstract 743.
Myelodysplastic Syndrome
Single cell network profiling (SCNP) reveals age- and disease-based heterogeneity in healthy individuals and in patients with low risk (LR) myelodysplastic syndrome (MDS).
Cohen AC, Huang Y-W, Cesano A, Hawtin RE, Ware JR, Arulrajah B, and Kornblau SM.
Blood (ASH Annual Meeting Abstracts). 2011;118:Abstract 2788.
Quantitative assessment of myeloid nuclear differentiation antigen distinguishes myelodysplastic syndrome from normal bone marrow.
Head HR, McClintock-Treep SA, Flye-Blakemore L, Jagasia M, Shinar A, Dupont WD, Stelzer G, Briggs R, and Shults KE.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 1754.
Immune System Monitoring
Single cell network profiling (SCNP) reveals race-associated differences in B cell receptor signaling pathway activation.
Longo D, Louie B, Mathi K, Pos Z, Wang E, Hawtin RE, Marincola F, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2011;118:Abstract 1125.
Immune system networks and their reorganization in rheumatoid arthritis.
Ptacek J, Hale MB, Khalili H, Batliwalla F, Aranow C, Mackay M, Diamond B, Gregersen PK, and Nolan GP.
International Immunology Meeting Abstracts. 2010;22(Suppl 1 Pt 2):PP-036-49.
Other
Single cell network profiling (SCNP) assay application to bladder cancer (BC) samples: Identifying rare epidermal growth factor (EGF) responsive epithelial cancer cells sensitive to PI3K inhibition from bladder washings.
Covey T, Vira M, Gulrajani M, Cholankeril M, Levey HR, Marimpietri C, Cesano A, and Fields SZ.
AACR International Conference: Molecular Targets and Cancer Therapeutics 2011 Nov 12-16; San Francisco, CA. Abstract # C92.
Cellular and intracellular network characterization of cytokine JAK/STAT signaling in whole blood across multiple healthy individuals: defining “normal”.
Covey TM, Gulrajani M, Marimpietri B, Chang AP, Panganiban-Lustan L, Cordeiro J, Longo D, Rosen D, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2010;116:Abstract 4768.